 |
Origin |
|
|
|
Peudomonas testosteroni |
|
| Preparation and Specification |
|
|
|
| Appearance |
: White amorphous pouder (Lyophilized powder) |
| Activity |
: More than 90 units/mg protein |
|
|
| Properties |
|
|
|
| Molecular weight |
: Approx. 37,000Da |
| Optimum pH |
: 10.2 ~ 10.5 |
| Optimum temp. |
: 50°C |
| pH stability |
: 6.0 ~ 9.5 (30°C, 17hr) |
| Thermal stability |
: below 50°C (pH7.2, 10min) |
| Michaelis constant |
: Androsterone |
6.7×10-6M |
|
: Na-cholate |
8.3×10-6M |
|
: NAD |
6.7×10-5M |
|
|
| Storage and Stability
|
|
|
|
| Below at 5°C |
: 1 year
: For prolonged storage, keep at -20°C |
| At room temp. |
: At least 1 week |
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|
| Contaminants |
|
|
|
| Malatedehydrogenase |
: below 0.01% |
| Alcoholdehydrogenase |
: below 0.01% |
| Lactatedehydrogenase |
: below 0.01% |
| ß-Hydroxysteroidedehydrogenase |
: below 0.5% |
|
|
| Unit definition |
|
|
|
One unit is the amount of enzyme required to oxidize 1µ mol
of androsteron as a substrate per minute in the presence of NAD at
pH 8.9 at 25°C. |
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| Package |
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|
 |
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| Activity assay principle |
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|
|
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| 100 units/bottle |
| 1,000 units/bottle |
|
|
|
|
-Hydroxysteroid
Dehydrogenase ( 3